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PhytoKeys 76: 31—38 (2017)

& doi: 10.3897/phytokeys.76. 1092 | 46Ph V toKe y S

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A new species of Erythrostemon (Leguminosae, Caesalpinioideae) from the western Rio Balsas Depression, Mexico

Solange Sotuyo', José Luis Contreras-Jiménez’, Gwilym P. Lewis?

| Departamento de Botanica, Instituto de Biologia, Universidad Nacional Auténoma de México. Circuito Exterior s/n, Ciudad Universitaria, Copilco, Coyoacdn. A.P 70-367 México, Distrito Federal. C.P 04510 2 Facultad de Arquitectura, Benemérita Universidad Auténoma de Puebla. 4 Sur 104. Col. Centro. CP 72000. Puebla, Puebla 3 Comparative Plant and Fungal Biology Department, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, U.K.

Corresponding author: Solange Sotuyo (jssotuyo@ib.unam.mx)

Academic editor: C. Morden | Received 25 October 2016 | Accepted 19 December 2016 | Published 9 January 2017

Citation: Sotuyo S, Contreras-Jiménez JL, Lewis GP (2017) A new species of Erythrostemon (Leguminosae, Caesalpinioideae) from the western Rio Balsas Depression, Mexico. PhytoKeys 76: 31-38. https://doi.org/10.3897/ phytokeys.76.10921

Abstract

A new legume species from a seasonally dry forest of the Western Rio Balsas Depression, in the states of Guer- rero and Michoacan, Mexico, Erythrostemon guevarafeferii, is herein described and illustrated. The new spe- cies shows morphological affinities with E. hintonii, from which it is distinguished in having fewer leaflets per pinna, mature leaflets disposed toward the upper half of the pinnae rachises, long inflorescences on curved slender peduncles, abundant red glands on its flowers and inflorescences, and its fruit glabrous with red stipi-

tate glands at maturity. A taxonomic key to the Rio Balsas Depression species of Erythrostemon is included.

Resumen

Una nueva especie de leguminosa de la Depresién Occidental del Rio Balsas en los estados de Guerrero y Michoacan, Erythrostemon guevarafeferii, se describe e ilustra. La nueva especie muestra afinidades mor- foldgicas con E. hintonii, de la que se distingue por tener menos pinnas, foliolos maduros glabros dispuestos hacia la mitad superior de las pinnas, las inflorescencias largas sobre peduinculos delgados que se curvan y flores con abundantes glandulas; legumbre glabra 0 con glandulas estipitadas rojas cuando esta madura. Se

incluye una clave taxondmica de las especies de Erythrostemon que habitan en la Depresién del Rio Balsas.

Keywords

Caesalpinia group, Leguminosae, Erythrostemon, Fabaceae, Neotropics

Copyright Solange Sotuyo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

32 Solange Sotuyo et al. / PhytoKeys 76: 31-38 (2017)

Introduction

Erythrostemon was re-circumscribed by Gagnon et al. (2016). The neotropical genus currently includes a total of 31 species of woody shrubs or small to medium-sized trees. Species distributions follow a bicentric amphitropical distribution pattern in México, Central America and the Caribbean, Brazil, Argentina, Bolivia, Chile and Paraguay (Lewis 1998; Gagnon et al. 2013; Gagnon et al. 2016). They grow in a wide range of habitats including seasonally dry tropical forests, caatinga vegetation, deserts, yungas- puna transition zones, and chaco-transition forests.

Species of Erythrostemon are distinctive by various combinations of several mor- phological traits (a reference for this cited here). Leaflets typically are either eglandular or have conspicuous black sessile glands along their margin that renders the margins slightly crenulated. Flowers have sepals that are ovate-lanceolate and their petals bend- ing and either yellow, red, pink or orange, the corolla sometimes laterally compressed. The androecium and gynoecium are free from the calyx and the ovary is eglandular or covered in gland-tipped trichomes. Legumes are typically oblong-elliptic pods with papery to slightly woody valves that are chartaceous or slightly woody, glabrous to pubescent, eglandular or with stipitate glands.

During a revision of Caesalpinia sensu lato in Guerrero and Michoacan, several spec- imens identified in herbaria as Caesalpinia hintonii Sandwith (a species recently trans- ferred to the genus Erythrostemon, Gagnon et al. 2016) proved to be morphologically distinct. Taking into account the above morphological diagnostic characters of Eryth- rostemon, a detailed study indicated that these specimens belong to that genus, although they do not match the description of any previously described species. The overall mor- phology of the new taxon most closely resembles Erythrostemon hintonii (Sandwith) E. Gagnon & G.P. Lewis, with which it shares a similar distribution area in the Rio Balsas Depression. The new species is herein described as Erythrostemon guevarafeferii.

Taxonomy

Erythrostemon guevarafeferii J.L.Contreras, S.Sotuyo & G.P.Lewis, sp. nov. urn:lsid:ipni.org:names:77159617-1 Figure 1

Type. México, Michoacan. Cerca de la cortina de la presa El Infiernillo /.L. Contreras 3111 (Holotype: MEXU; Isotypes: FCME, K, MEXU).

Erythrostemon hintonii affinis sed glabra, structuris floralibus omnino glanduliferis, foliis minoribus cum minus foliolis fasciculatus in dimidio superiore rhachidis pinnae, floribus brevioribus, sepalis laete ochraceis et petalis salmoneis, pedicellis articulatis in dimidio superiore longitudinis, legumine omnino glabro et stipitatis glandulis rubris obducto. Etiam differt inflorescentias arcuatas et pendulas cum pedicellis florum gra- cilibus, fere horizontalibus vel reflexis, tortis itaque floribus resupinatis.

A new species of Erythrostemon (Leguminosae, Caesalpinioideae)... 33

Figure |. Evythrostemon guevarafeferii, a bipinnate leaves b leaflet upper surface showing marginal glands C inflorescence with immature fruits developing at base d flower e adaxial (median) petal f lateral petal g abaxial petal h gynoecium i stamens j calyx k fruit showing the red stipitate glands as black dots. (a-j drawn from Contreras-Jiménez et al. 3111, holotype MEXU k from Contreras-Jiménez 2860). Drawn by Albino Luna.

34 Solange Sotuyo et al. / PhytoKeys 76: 31-38 (2017)

Similar to Erythrostemon hintonii, but glabrous, all flower structures glandular, leaves smaller with fewer leaflets, flowers shorter, sepals light yellow-ochre and petals salmon coloured, pedicels articulated at, or above, their middle, legume completely glabrous and with stipitate red glands. Also differing in the curved and/or pendulous inflorescences with the flowers on slender, horizontal or reflexed, twisted pedicels that render them resupinate.

Description. Small tree or shrub, 2—6 m tall, bark grey pruinose, exfoliating, young branches reddish grey pruinose. Leaves bipinnate, (7.5—) 10-20 (—22) cm long; stipules triangular-acuminate, caducous, 2—3 x 1.2—1.5 mm, margin with red stipitate glands, white hairy on both surfaces; petiole (2—) 3.0—6.5 (—7.3) cm long, pubescent or glabrescent when mature; rachis (3—) 5.0-14.5 (—15.7) cm long, indu- mentum similar to that of the petiole; pinnae (5—) 7-13 per leaf, (1.6—) 2-6 (-7.7) cm long, densely pubescent when young, glabrescent when mature; leaflets (3) 4-7 (—10) jugate, clustered on the upper half of the pinnae rachises, obovate, elliptic or oblong-elliptic (6—) 10—20 (—25) x (4) 5-11 (-13) mm, base rounded, slightly oblique, margin entire and lacking glands, or with sunken black punctate glands that render the margin slightly crenulate, apex obtuse to rounded, pubescent or glabres- cent at maturity. Inflorescence an erect or pendulous (the peduncles curved down- wards), terminal or axillary raceme or panicle (with few branches near the base), (6—) 10-36 cm long, densely white pubescent, and with red glands; bracts ovate-caudate, caducous, (2.5—) 3.2-5.0 (—5.7) x (1-) 1.3-2.0 (2.3) mm, margin with or without glands, densely white pubescent on both surfaces; pedicels slender, erect-patent, (6—) 7.5-11.0 (—16.5) mm long, articulated at or above the middle, densely white pubes- cent, sometimes twisted, so that the flowers are resupinate (although then presented with the median petal uppermost on the pendulous inflorescences). Calyx light yel- low-ochre, densely pubescent, tube obliquely obconic, laterally compressed, (4.3-) 4,.7-6.0 x (2.1—) 2.5-3.5 (4) mm; adaxial sepals ovate to oblong-ovate, 5.5—7.0 x 2.5—4.1 mm, slightly concave, the lateral sepals oblong-ovate, 5.4—7.0 mm x 2.7—4.0 mm; abaxial sepal cymbiform, (5.7—) 6—7.2 (—7.4) x (3.6—) 3.8-5.0 mm, all sepals with an entire margin, glandular ciliate and with an acute apex, puberulent on adaxial surface; petals salmon-coloured, clawed; adaxial petal ovate, 5.2—7.5 x 3.5-5.5 mm, base cordate, margin entire, apex obtuse, rounded, with stipitate glands near the claw apex on the abaxial surface, pilose on the adaxial surface; claw 1.8—3.0 mm long, sigmoid curved, ciliate, pilose and glandular-stipitate on abaxial surface or glabrous and sparsely stipitate-glandular; lateral petals ovate, 5.6—7.5 (—8) x 4.2—6 (—6.5) mm, base obtuse, margin glandular ciliate up to % of its length, apex rounded or obtuse, stipitate-glandular on abaxial surface, the abaxial petals ovate (6—) 6.5-8.5 x 3.7—5.5 (—5.7) mm, base oblique, margin entire, glandular ciliate from the base up to ¥% of their length, apex obtuse, the claw ciliate and pilose on its abaxial surface; stamens curved, filaments (6.5—) 7.5—9.5 (-10) mm long, flattened at the base, densely vil- lous to % of their length, the upper third with lime green stipitate glands; anthers oblong-elliptic, (1.2—) 1.3-1.8 x (0.8—-) 0.9-1.2 (—1.3) mm, erect at anthesis; ovary

A new species of Erythrostemon (Leguminosae, Caesalpinioideae)... 35

(1.7—) 2-3 (-3.5) mm long borne on a stipe 0.5 mm long, densely sericeous and with green cupuliform glands (or these absent); style curved, of different lengths in individual flowers, either short, 2.5—3.5 mm long, or well developed and 4.0—6.5 mm long, pilose for 12 of its length from base; stigma porate laterally; ovules 2 (—3) per ovary. Legume falcate, (3.7—) 4.3-5.7 x 1.1-1.7 cm, chartaceous, brown-yellow to vinaceous, densely to sparsely pubescent or glabrous when mature, with red stipitate glands or these glands absent, sutures densely pubescent, elastically dehiscent; seeds 1—2 (3) per fruit, obovate, (8.6—) 9.5-10.5 (-11) x 7.5-9.6 x (1.7—) 1.9-2.2 (—2.4) mm, olive-brown, nitid.

Habitat. Seasonally dry tropical forest on rocky slopes, locally common in second- ary vegetation along roads, on alluvial soils near seasonal or permanent streams.

Distribution and phenology. Known only from the western region of the Rio Balsas Depression, in the states of Guerrero and Michoacan. Flowering from February to April and in fruit from February to May.

Selected specimens examined. MEXICO. Michoacan: Municipio de Arteaga: El Infiernillo, cerca de la cortina de la presa: Nunez & Silva 3905 (MEXU); J.C. Soto 1331 eS. Zarate (MEXU)); J.C. Soto 3694 (MEXU); Sotuyo et al. 41,46,47,48 (K, MEXU); 23 km por el camino a Infiernillo a partir de la carretera Apatzingan-Lazaro Cardenas: J.L. Contreras 2060, 2838 (MEXU). Municipio de Nocupétaro: San Antonio de los Muertos; J.C. Soto 3905 & G. Silva (MEXU). Guerrero: Municipio de la Unidén: 5 km al S de Colmeneros, camino a Coahuayutla: /.L. Contreras 2371, 2372 (FCME); Cerro Prieto, 12 km al E de la Garita: /.L. Contreras 2388 (FCME); Zihuatanejo, 85 km aprox. NW, on road 22 km to N of La Unién and 7 km to N of Las Juntas del Rio towards Santa Maria: D.J. Macqueen & A. Nileshwar 446 (K).

IUCN Red List category. We recommend that Erythrostemon guevarafeferii be given a conservation assessment of Vulnerable [VU (B1b-iii)], in accordance with IUCN (2012) categories and criteria. The extent of occurrence (EOO) of E. gue- varafeferii is estimated to be over 2424.18 km’, well below the 20,000 km? upper limit for Vulnerable status under criterion B1, but its area of occupancy (AOO) is estimated to be less than 10 km* (the limit for Endangered status under criterion B2). The species is currently known from three discontinuous populations in the states of Michoacan and Guerrero, these separated by differing habitat type, human settlement and agricultural land. Erythrostemon guevarafeferii is known in only one protected area, the Reserva de la Biosfera Zicuiran-Infiernillo in Michoacan. The preferred habitat of the species is potentially threatened by future settlement and agricultural activities, as well as by environmental problems associated with drug traficking organizations.

Etymology. The species epithet is dedicated to Fernando Guevara Fefer who re- cently passed away. Friend, botany teacher and researcher at the Universidad Michoa- cana de San Nicolas de Hidalgo, he was interested in the genus Bursera and in floristic and vegetation studies within Michoacan, particularly in the Infiernillo region where the type specimen was collected.

36 Solange Sotuyo et al. / PhytoKeys 76: 31-38 (2017)

Discussion

Erythrostemon guevarafeferii differs from Erythrostemon hintonii in being glabrous at ma- turity and by sometimes having glands on floral structures and fruits. It has smaller leaves with (5—) 7-13 pinnae per leaf and leaflets (3—) 4-7 (—10) jugate, these on the upper half of the pinna rachis. Flowers are smaller (1.2—1.6 cm long, including the calyx) with light yellow-ochre sepals and salmon-pink petals, its pedicels are articulated at or above their middle. Its legume is glabrous when mature and sometimes has red stipitate glands.

Erythrostemon hintonii differs by having leaves with 3-9 pinnae per leaf and leaflets 4—6 jugate, these distributed along the length of the pinna rachis, flowers 2 cm long (including the calyx) with red-purple sepals and purple-red petals, pedicels that are articulated near the base of the calyx tube, and a legume that is pubescent and has dark red-brown sessile glands. Some individual plants of E. guevarafeferii have glandular fruits while others in the same locality do not. The species is characterized by its arched and pendulous inflorescences, with slender, nearly horizontal pedicels (or these reflexed and sometimes twisted, so that the flowers are resupinate). Resupinate flowers on a pendulous inflorescence renders them, once again, in their usual position with the median (standard) petal uppermost.

Field and laboratory observations reveal two kinds of flower, with either a short or long pistil, as also observed in Erythrostemon epifanioi (J.L. Contr.) E. Gagnon & G.P. Lewis (as Caesalpinia epifanioi J.L. Contr.; Lewis, 1998) and in another legume genus, 7ylosema esculentum (Burch.) A. Schreib., which displays functional hetero- styly (Hartley et al. 2002). Flowers with short pistils are functionally male and flow- ers with long pistils are hermaphrodite. We hypothesise that the occurrence of both flower forms in the same inflorescence ensures that not too many flowers on each inflorescence set fruit (which would put a strain on maternal resources, as well as me- chanically over-loading the peduncle) and that pollinators continue to move pollen throughout or between populations of the species, thus promoting cross-pollination. Andromonoecy, with a labile sex change of flowers from hermaphrodite to function- ally male (the gynoecium development is suppressed in flowers higher up an inflo- rescence only if the lower flowers are successfully pollinated and fruits are set) has been observed in Erythrostemon calycina (Benth.) L.P. Queiroz (as Caesalpinia calycina Benth.) in Brazil (Gibbs et al. 1999).

Taxonomic key to the species of Erythrostemon in Rio Balsas Depression

| Shrubs or herbaceous plants; leaflets without black glands along margin; flowers deflexed; glandular stamen filaments curved, twice the length of the petals; legume 4.7—7.1 cm long; Sierra Madre del Sur, Guerrero and Oaxaca, TOO =2 OOO smncelevatt@ Mies h..27 vee cise Ede deans lsnsulacctanteteteeacs ates E. laxus = Trees; leaflets with black glands along margin; flowers reflexed; stamen fila- ments falcate or curved, equalling the length of the petals; legume 3—6 cm long; seasonally dry forest of the Rio Balsas Depression, 100-1200 m elevation..... 2

A new species of Erythrostemon (Leguminosae, Caesalpinioideae)... ae

2 Leaves with 3—5 pairs per pinnae, leaflets 2—6 pairs per pinna; inflorescence a TAGEME; OMA: SHOLE WOOGY_DEACI VAST Ny, yates tanstedenns ead read dors ersneeetaueigaat yes 3 - Leaves with 7—13 pairs per pinnae, leaflets 4—11 pairs per pinna; inflorescence a terminal or axillary raceme or panicle, peduncles branched near base....... 4 3 Leaflets 2—3 pairs per pinna; pedicels 6.5—12.5 mm long, pubescent; eastern region of Rio Balsas Depression (Ozomatlan, Guerrero)............ E. epifanioi ms Leaflets 4—6 pairs per pinna; pedicels c. 7 mm long, densely stipitate-glandu- lar; Valle de Tehuacan-Cuicatlan (Puebla and Oaxaca)......E. melanadenius 4 Pedicels articulated below the middle; petals yellow; leaflets oblong-elliptic.... Fiatedseponta ths cers ates dBc betung dUicle aes:t doen ehdvs Seenceuad tundeneadeda it eiseessees E. macvaughii = Pedicels articulated at or above the middle; petals pink, red or scarlet; leaflets OVALE LO LODGING io. het bidioutyahpretisrchands ichaiedenemmnictesmedsrmnnitetnntimamuadt yas dnl wap etait 5 5 Fruit with red cupuliform glands or these absent; standard petal red or salmon coloured, stamen filaments pubescent on basal 12 to 34, sometimes stipitate- glandular; western part of Rio Balsas Depression (Guerrero and Michoacan).. 6 — Fruit with green pixie-cup (or doughnut-shaped) glands; standard petal pale pink with a red blotch at the lamina base on the inner surface; stamen fila- ments with green glands; western Rio Balsas Depression (Guerrero, Oaxaca alt Purely) eissi ti seca veces she ahead Se toc ante ioulnadceduee Raav deg eteecnenildas E. oyamae 6 Leaflets obovate to elliptic, inserted along the pinna rachis; bracts erect or incurved; stamen filaments 13-15 mm long; legume with red cupuliform glands; seasonally dry forest in Guerrero and Michoacan............ E. hintonii = Leaflets oblong-elliptic, inserted on the upper half of the pinna rachis; bracts ovate-caudate; stamen filaments 6.5-10 mm long; legume with or without red cupuliform glands; Infiernillo region in the Rio Balsas Depression (Guer- Pero and MMichOaeaIt) ci ehh ovate caendingas. Suiaksau at E. guevarafeferii

Acknowledgments

We thank Albino Luna for the botanical illustration and Martha Martinez Gordillo for the Latin diagnosis. We thank the editor and two anonymous reviewers for their constructive comments, which helped us to improve the manuscript.

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